The scientific project of the Quantitative Image Analysis (QuIA) unit is to develop image analysis and computer vision tools for the processing and quantification of biological images, with a strong focus on multi-channel temporal 3D sequences. Our work over the last years has been centered on developing new algorithms for multi-particle tracking, active contours models, PSF approximations for image deconvolution and colour image analysis. It has resulted in powerful tools for spot detection and counting in real-time imaging of virus and genes, movement and shape analysis in 3D+t microscopy and histological biopsies analysis. These methods and algorithms are made available to biological groups with which we collaborate in a large number of projects.